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tbx5 protein  (Human Protein Atlas)

 
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    Human Protein Atlas tbx5 protein
    Tbx5 Protein, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbx5 protein/product/Human Protein Atlas
    Average 90 stars, based on 1 article reviews
    tbx5 protein - by Bioz Stars, 2026-02
    90/100 stars

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    (A) Three TMCO1 SNPs fall within a predicted <t>TBX5</t> binding motif. (B) EMSA result for variants rs4657475, rs116461237 and rs61058666. Lane 1, Nppa promoter is known to bind to TBX5. Lane 2, wildtype SNP sequence forms complex with TBX5. Lanes 4–7, SNP rs116461237 variant disrupts TBX5 binding. Lanes 8–9, TBX5 does not form DNA-protein complex with Alu consensus sequence. Lanes 10–12, mutation of rs116461237 only disrupts TBX5-probe complex formation. Lanes 3, 7 and 9 contain cold competitor in addition to probe sequence. SNP, single nucleotide polymorphism.
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    tbx5 protein  (Human Protein Atlas)
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    (A) Three TMCO1 SNPs fall within a predicted <t>TBX5</t> binding motif. (B) EMSA result for variants rs4657475, rs116461237 and rs61058666. Lane 1, Nppa promoter is known to bind to TBX5. Lane 2, wildtype SNP sequence forms complex with TBX5. Lanes 4–7, SNP rs116461237 variant disrupts TBX5 binding. Lanes 8–9, TBX5 does not form DNA-protein complex with Alu consensus sequence. Lanes 10–12, mutation of rs116461237 only disrupts TBX5-probe complex formation. Lanes 3, 7 and 9 contain cold competitor in addition to probe sequence. SNP, single nucleotide polymorphism.
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    (A) Three TMCO1 SNPs fall within a predicted <t>TBX5</t> binding motif. (B) EMSA result for variants rs4657475, rs116461237 and rs61058666. Lane 1, Nppa promoter is known to bind to TBX5. Lane 2, wildtype SNP sequence forms complex with TBX5. Lanes 4–7, SNP rs116461237 variant disrupts TBX5 binding. Lanes 8–9, TBX5 does not form DNA-protein complex with Alu consensus sequence. Lanes 10–12, mutation of rs116461237 only disrupts TBX5-probe complex formation. Lanes 3, 7 and 9 contain cold competitor in addition to probe sequence. SNP, single nucleotide polymorphism.
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    (A) Three TMCO1 SNPs fall within a predicted TBX5 binding motif. (B) EMSA result for variants rs4657475, rs116461237 and rs61058666. Lane 1, Nppa promoter is known to bind to TBX5. Lane 2, wildtype SNP sequence forms complex with TBX5. Lanes 4–7, SNP rs116461237 variant disrupts TBX5 binding. Lanes 8–9, TBX5 does not form DNA-protein complex with Alu consensus sequence. Lanes 10–12, mutation of rs116461237 only disrupts TBX5-probe complex formation. Lanes 3, 7 and 9 contain cold competitor in addition to probe sequence. SNP, single nucleotide polymorphism.

    Journal: The British Journal of Ophthalmology

    Article Title: SNP located in an AluJb repeat downstream of TMCO1, rs4657473, is protective for POAG in African Americans

    doi: 10.1136/bjophthalmol-2018-313086

    Figure Lengend Snippet: (A) Three TMCO1 SNPs fall within a predicted TBX5 binding motif. (B) EMSA result for variants rs4657475, rs116461237 and rs61058666. Lane 1, Nppa promoter is known to bind to TBX5. Lane 2, wildtype SNP sequence forms complex with TBX5. Lanes 4–7, SNP rs116461237 variant disrupts TBX5 binding. Lanes 8–9, TBX5 does not form DNA-protein complex with Alu consensus sequence. Lanes 10–12, mutation of rs116461237 only disrupts TBX5-probe complex formation. Lanes 3, 7 and 9 contain cold competitor in addition to probe sequence. SNP, single nucleotide polymorphism.

    Article Snippet: EMSA was performed using Biotinylated oligonucleotide probes ( ) and purified recombinant protein of human T-box 5 (TBX5) with C-terminal MYC/DDK tag (Origene, Rockville, MD; TP316520) and the Odyssey Infrared EMSA kit (LiCor, Lincoln, Nebraska; 829–07910).

    Techniques: Binding Assay, Sequencing, Variant Assay, Mutagenesis